Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriers

• Dong Ye,
• Mattia Bramini,
• Delyan R. Hristov,
• Sha Wan,
• Anna Salvati,
• Christoffer Åberg and
• Kenneth A. Dawson

Beilstein J. Nanotechnol. 2017, 8, 1396–1406, doi:10.3762/bjnano.8.141

• transport through them. Keywords: Caco-2; differentiation and polarisation; epithelial cell barrier; microscopy imaging; particle interaction; uptake and localisation; Introduction An overall conclusion from a multitude of nanoparticle-cell in vitro studies is that nanoparticle uptake into cells is

• fluorescence decreases during the 4 h without nanoparticles present, suggesting that the association of nanoparticles with cells is (at least partly) only transient. To better understand the observed behaviour and investigate further particle uptake and localisation under the different conditions, we turned to